doi: 10.1073/pnas.73.4.1053.
Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro
- PMID: 772677
- PMCID: PMC430198
- DOI: 10.1073/pnas.73.4.1053
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Involvement of escherichia coli dnaZ gene product in DNA elongation in vitro
Proc Natl Acad Sci U S A.
1976 Apr.
Abstract
E. coli dnaZ gene product is required for conversion of phiX174, fd, and ST-1 single-stranded phage DNAs to duplex DNAs in vitro. This protein has been purified about 5000-fold. It functions in the elongation of RNA- or DNA-primed single-stranded DNA that is catalyzed by DNA polymerase III(DNA nucleotidyltransferase; deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase; EC 2.7.7.7) in conjunctions with two other E. coli protein preparations referred to as DNA elongation factors I and III. It also functions in similar reactions catalyzed by DNA polymerase II in combination with E. coli DNA binding protein and DNA elongation factors I and III.
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