doi: 10.1016/0014-5793(95)00278-h.
The thrombin receptor in human platelets is coupled to a GTP binding protein of the G alpha q family
Affiliations
- PMID: 7729552
- DOI: 10.1016/0014-5793(95)00278-h
Item in Clipboard
The thrombin receptor in human platelets is coupled to a GTP binding protein of the G alpha q family
FEBS Lett.
.
Free article
Abstract
The thrombin receptor is a G protein-coupled receptor, but the G proteins functionally coupled to this receptor in human platelets are not yet definitively identified. Thrombin stimulation of platelets leads to phospholipase C-mediated increases in intracellular calcium, and previous studies have suggested that the thrombin receptor is coupled to members of the Gq family. We now demonstrate direct GTPase activation by thrombin receptor activation peptide (TRAP) in human platelet membranes, and specific inhibition of TRAP-activated GTPase by antibodies to Gq. These data demonstrate functional coupling of the thrombin receptor to a member of the Gq family.
Similar articles
-
Thrombin receptor activation by thrombin and receptor-derived peptides in platelet and CHRF-288 cell membranes: receptor-stimulated GTPase and evaluation of agonists and partial agonists.Mol Pharmacol. 1996 Jan;49(1):190-7. Mol Pharmacol. 1996. PMID: 8569706
-
Thromboxane-insensitive dog platelets have impaired activation of phospholipase C due to receptor-linked G protein dysfunction.J Clin Invest. 1993 Nov;92(5):2469-79. doi: 10.1172/JCI116855. J Clin Invest. 1993. PMID: 8227362 Free PMC article.
-
G proteins of the G12 family are activated via thromboxane A2 and thrombin receptors in human platelets.Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):504-8. doi: 10.1073/pnas.91.2.504. Proc Natl Acad Sci U S A. 1994. PMID: 8290554 Free PMC article.
-
Agonist receptors and G proteins as mediators of platelet activation.Adv Exp Med Biol. 1993;344:17-36. doi: 10.1007/978-1-4615-2994-1_2. Adv Exp Med Biol. 1993. PMID: 8209785 Review.
-
The thrombin receptor.Clin Exp Pharmacol Physiol. 1994 May;21(5):349-58. doi: 10.1111/j.1440-1681.1994.tb02527.x. Clin Exp Pharmacol Physiol. 1994. PMID: 7955544 Review.
Cited by
-
Platelet signal transduction defect with Galpha subunit dysfunction and diminished Galphaq in a patient with abnormal platelet responses.Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8750-5. doi: 10.1073/pnas.94.16.8750. Proc Natl Acad Sci U S A. 1997. PMID: 9238049 Free PMC article.
-
N-linked glycosylation of protease-activated receptor-1 second extracellular loop: a critical determinant for ligand-induced receptor activation and internalization.J Biol Chem. 2010 Jun 11;285(24):18781-93. doi: 10.1074/jbc.M110.111088. Epub 2010 Apr 5. J Biol Chem. 2010. PMID: 20368337 Free PMC article.
-
Protein kinase C- and calcium-regulated pathways independently synergize with Gi pathways in agonist-induced fibrinogen receptor activation.Biochem J. 2002 Dec 1;368(Pt 2):535-43. doi: 10.1042/BJ20020226. Biochem J. 2002. PMID: 12215172 Free PMC article.
-
Signalling functions and biochemical properties of pertussis toxin-resistant G-proteins.Biochem J. 1997 Feb 1;321 ( Pt 3)(Pt 3):561-71. doi: 10.1042/bj3210561. Biochem J. 1997. PMID: 9032437 Free PMC article. Review.
-
Protease Activated Receptors and Arthritis.Int J Mol Sci. 2021 Aug 28;22(17):9352. doi: 10.3390/ijms22179352. Int J Mol Sci. 2021. PMID: 34502257 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
