Differentiation by pyridoxal 5-phosphate, PPADS and IsoPPADS between responses mediated by UTP and those evoked by alpha, beta-methylene-ATP on rat sympathetic ganglia

Abstract

1. The effect of pyridoxal 5-phosphate, and the 2',4' and 2',5'-disulphonic acid isomers of 6-azophenylpyridoxal 5-phosphate (PPADS and IsoPPADS respectively) on depolarization of the rat superior cervical ganglion evoked by alpha, beta-methylene-adenosine 5'-triphosphate (alpha, beta-Me-ATP) and uridine 5'-triphosphate (UTP) were determined by a grease-gap recording technique. 2. Pyridoxal 5-phosphate (10-100 microM) and PPADS (10-100 microM) enhanced UTP- and depressed alpha, beta-Me-ATP-evoked depolarizations but did not significantly alter depolarizations evoked by potassium or hyperpolarizations evoked by adenosine. IsoPPADS (10 microM) depressed alpha, beta-Me-ATP-evoked depolarizations but did not alter depolarizations evoked by UTP. Depolarizations evoked by muscarine were depressed by IsoPPADS but not by pyridoxal 5-phosphate. 3. It is concluded that pyridoxal 5-phosphate, PPADS and IsoPPADS are antagonists at P2x-purinoceptors but not at the receptors that mediate UTP-evoked depolarization of the rat superior cervical ganglion. These observations substantiate the recent proposal that the rat superior cervical ganglia possess distinct receptors for purine and pyrimidine 5'-nucleotides, i.c. P2x-purinoceptors and pyrimidinoceptors respectively.