ATP stimulated catecholamine secretion: response in perfused adrenal glands and a subpopulation of cultured chromaffin cells

Abstract

Extracellular ATP is shown to induce catecholamine secretion in bovine chromaffin cells. Our data indicate that cells in culture gradually increase their response to ATP, and we have separated freshly isolated cells on a density gradient and found that the lighter cells develop a much stronger response to ATP than do the heavier cells. To see if the ATP sensitivity is physiological, we have perfused intact adrenal glands. ATP induces a greater secretory response from glands than does acetylcholine without causing preferential secretion of norepinephrine or epinephrine. These data show that the response to ATP found in cultured cells is not an artifact of cell culture, and that ATP co-released with catecholamines from the storage vesicles may have a significant physiological role.