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Comparative Study
. 1994 Jun;32(6):1591-3.
doi: 10.1128/jcm.32.6.1591-1593.1994.

Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing

G E Killgore  1 , H Kato
Affiliations
Comparative Study

Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing

G E Killgore et al. J Clin Microbiol. 1994 Jun.

Abstract

An arbitrarily primed PCR (AP-PCR) assay was used to type Clostridium difficile isolates from a hospital outbreak of antibiotic-associated diarrhea. Forty-one isolates were separated into nine groups, with 66% falling into one group; no other group contained more than 10%. Comparison of AP-PCR grouping with that when the immunoblot technique was used showed agreement for 33 of 34 isolates typed by both techniques, and AP-PCR grouped seven isolates that were not typeable by immunoblotting.

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Comment in

  • Genotyping of Clostridium difficile isolates.
    Gürtler V, Mayall BC. Gürtler V, et al. J Clin Microbiol. 1994 Dec;32(12):3095. doi: 10.1128/jcm.32.12.3095-.1994. J Clin Microbiol. 1994. PMID: 7533782 Free PMC article. No abstract available.

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