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Comparative Study
. 1995 Apr 26;1261(3):424-6.
doi: 10.1016/0167-4781(95)00040-n.

Molecular cloning and expression of human ribonuclease 4 cDNA

M Seno  1 , J Futami, Y Tsushima, K Akutagawa, M Kosaka, H Tada, H Yamada
Affiliations
Comparative Study

Molecular cloning and expression of human ribonuclease 4 cDNA

M Seno et al. Biochim Biophys Acta. .

Abstract

A cDNA coding for human ribonuclease 4 was isolated from a pancreas cDNA library and sequenced. This cDNA (996 bp) includes an entire open reading frame encoding mature protein (119 aa) following signal peptide (28 aa). Expression of mature protein in Escherichia coli showed an apparent molecular mass of about 16 kDa, which was slightly lower than the mature form of human RNase 1, in SDS-PAGE.

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