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. 1994 Dec;27(1):26-30.
doi: 10.1007/BF00326575.

High-efficiency electrotransformation of the yeast Schwanniomyces occidentalis

P Costaglioli  1 , E Meilhoc, J M Masson
Affiliations

High-efficiency electrotransformation of the yeast Schwanniomyces occidentalis

P Costaglioli et al. Curr Genet. 1994 Dec.

Abstract

A method has been developed for introducing heterologous DNA rapidly and efficiently by electropermeabilization into the yeast Schwanniomyces occidentalis. A transformation efficiency as high as 2 x 10(5) transformants/microgram of plasmid DNA was obtained with a square-wave electric pulse of 2.17 kV/cm during 18 ms. Small quantities of DNA (5 ng) can be used to transform 3 x 10(8) cells. The main parameters which have been optimized are: presence of adenine in the culture medium, pretreatment of the cells with dithiothreitol during the exponential growth phase of the cells, amount of cells treated, and pulse-field strength and duration. Competent cells can be stored to allow electrotransformation whenever needed.

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