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. 1995 Mar;33(3):629-30.
doi: 10.1128/jcm.33.3.629-630.1995.

Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin

M Amicosante  1 , L Richeldi, G Trenti, G Paone, M Campa, A Bisetti, C Saltini
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Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin

M Amicosante et al. J Clin Microbiol. 1995 Mar.

Abstract

Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).

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