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. 1995 Mar;33(3):725-8.
doi: 10.1128/jcm.33.3.725-728.1995.

Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA

R D Allen  1 , P E Pellett, J A Stewart, M Koopmans
Affiliations

Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA

R D Allen et al. J Clin Microbiol. 1995 Mar.

Abstract

We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.

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References

    1. J Infect Dis. 1975 Nov;132(5):568-77 - PubMed
    1. J Infect Dis. 1988 Dec;158(6):1177-84 - PubMed
    1. J Clin Microbiol. 1989 Jun;27(6):1238-42 - PubMed
    1. J Clin Microbiol. 1989 Aug;27(8):1802-9 - PubMed
    1. J Clin Microbiol. 1990 Mar;28(3):495-503 - PubMed