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. 1976 Mar 22;144(2):217-21.
doi: 10.1007/BF02428112.

The maintenance affinities of KLF-1 proximal F merogenotes in Escherichia coli

J Habácek, H Braná

The maintenance affinities of KLF-1 proximal F merogenotes in Escherichia coli

J Habácek et al. Mol Gen Genet. .

Abstract

In conjugation with donor strains carrying proximal F merogenotes of KLF-1 type about 100-fold lower frequency of Leu+ or Lac+ recombinants was found. The determination of the level of beta-galactosidase synthesis during the initial period of mating indicated that the transfer process of plasmid DNA was not impaired. Among the recombinants selected a large fraction have not expressed the plasmic fertility functions. This phenomenon was found to be replicon specific and was observed only with proximal F merogenotes but not with classical F'lac and F'ORF-1 elements or R1-19 plasmid. The expression of KLF-1 plasmid functions in the cell seems to be affected by a chromosomal gene of the proximal F merogenote closely linked to leu marker.

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