Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Mar 22;144(2):217-21.
doi: 10.1007/BF02428112.

The maintenance affinities of KLF-1 proximal F merogenotes in Escherichia coli

J Habácek, H Braná

The maintenance affinities of KLF-1 proximal F merogenotes in Escherichia coli

J Habácek et al. Mol Gen Genet. .

Abstract

In conjugation with donor strains carrying proximal F merogenotes of KLF-1 type about 100-fold lower frequency of Leu+ or Lac+ recombinants was found. The determination of the level of beta-galactosidase synthesis during the initial period of mating indicated that the transfer process of plasmid DNA was not impaired. Among the recombinants selected a large fraction have not expressed the plasmic fertility functions. This phenomenon was found to be replicon specific and was observed only with proximal F merogenotes but not with classical F'lac and F'ORF-1 elements or R1-19 plasmid. The expression of KLF-1 plasmid functions in the cell seems to be affected by a chromosomal gene of the proximal F merogenote closely linked to leu marker.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1964 Feb;51:351-9 - PubMed
    1. Mol Gen Genet. 1973 Dec 31;127(4):297-306 - PubMed
    1. J Mol Biol. 1970 May 28;50(1):111-27 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 - PubMed
    1. Mol Gen Genet. 1974;135(2):131-47 - PubMed

LinkOut - more resources