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. 1976 Mar 22;144(2):223-30.
doi: 10.1007/BF02428113.

Genetic and biochemical studies of phosphomannose isomerase deficient mutants of Saccharomyces cerevisiae

L S Herrera, C Pascual, X Alvarez

Genetic and biochemical studies of phosphomannose isomerase deficient mutants of Saccharomyces cerevisiae

L S Herrera et al. Mol Gen Genet. .

Abstract

Three mannose-negative mutants of Saccharomyces cerevisiae have been isolated. These mutants showed growth inhibition when mannose was added to a growth medium containing glycerol or fructose. Crosses between wild type mutants showed segregation of 2+/2-. Crosses between the mutants themselves showed that they were closely linked. Two mutants (XM3 and D2) showed characteristics of allelic structural alteration of phosphomannoseisomerase. Mutant D4 had a deficiency of phosphomannoseisomerase activity, but with a normal thermostability. Revertants from D4 had a normal thermostability.

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