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. 1995 May 9;92(10):4572-6.
doi: 10.1073/pnas.92.10.4572.

Plasmid DNA entry into postmitotic nuclei of primary rat myotubes

M E Dowty  1 , P Williams, G Zhang, J E Hagstrom, J A Wolff
Affiliations

Plasmid DNA entry into postmitotic nuclei of primary rat myotubes

M E Dowty et al. Proc Natl Acad Sci U S A. .

Abstract

These studies were initiated to elucidate the mechanism of DNA nuclear transport in mammalian cells. Biotin- or gold-labeled plasmid and plasmid DNA expression vectors for Escherichia coli beta-galactosidase or firefly luciferase were microinjected into the cytoplasm of primary rat myotubes in culture. Plasmid DNA was expressed in up to 70% of the injected myotubes, which indicates that it entered intact, postmitotic nuclei. The nuclear transport of plasmid DNA occurred through the nuclear pore by a process common to other large karyophilic macromolecules. The majority of the injected plasmid DNA was sequestered by cytoplasmic elements. This understanding of plasmid DNA nuclear transport provides a basis for increasing the efficiency of gene transfer.

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References

    1. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4438-42 - PubMed
    1. Nature. 1993 Oct 14;365(6447):666-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6094-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7333-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2702-6 - PubMed

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