Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 May 12;268(5212):877-80.
doi: 10.1126/science.7754372.

Gene trap tagging of PROLIFERA, an essential MCM2-3-5-like gene in Arabidopsis

P S Springer  1 , W R McCombie, V Sundaresan, R A Martienssen
Affiliations

Gene trap tagging of PROLIFERA, an essential MCM2-3-5-like gene in Arabidopsis

P S Springer et al. Science. .

Abstract

Gene trap transposon mutagenesis can identify essential genes whose functions in later development are obscured by an early lethal phenotype. In higher plants, many genes are required for haploid gametophyte viability, so that the phenotypic effects of their disruption cannot be readily observed in the diploid plant body. The PROLIFERA (PRL) gene, identified by gene trap transposon mutagenesis in Arabidopsis, is required for megaga-metophyte and embryo development. Reporter gene expression patterns revealed that PRL was expressed in dividing cells throughout the plant. PRL is related to the MCM2-3-5 family of yeast genes that are required for the initiation of DNA replication.

PubMed Disclaimer

Publication types

MeSH terms

Associated data