Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 May 19;81(4):601-9.
doi: 10.1016/0092-8674(95)90081-0.

Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor

Y Kubota  1 , S Mimura, S Nishimoto, H Takisawa, H Nojima
Affiliations
Free article

Identification of the yeast MCM3-related protein as a component of Xenopus DNA replication licensing factor

Y Kubota et al. Cell. .
Free article

Abstract

Replication licensing factor is thought to be involved in the strict control of the initiation of DNA replication in eukaryotes. We identified a 100 kDa protein as a candidate for the licensing factor in Xenopus egg extracts. This protein was required for replication; it bound to sperm DNA before the formation of nuclei and apparently dissociated from the nuclear DNA during the progression of replication without being transported into the nuclei. An immunologically homologous protein in HeLa cells behaved similarly to the Xenopus protein during the cell cycle. Cloning and sequencing of the cDNAs encoding the Xenopus and human proteins revealed that they are homologs of yeast Mcm3, a putative yeast DNA replication licensing factor.

PubMed Disclaimer

Publication types

Associated data