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. 1995 Apr 24;156(2):253-7.
doi: 10.1016/0378-1119(95)00035-5.

Cloning of ascidian homeobox genes provides evidence for a primordial chordate cluster

A Di Gregorio  1 , A Spagnuolo, F Ristoratore, M Pischetola, F Aniello, M Branno, L Cariello, R Di Lauro
Affiliations

Cloning of ascidian homeobox genes provides evidence for a primordial chordate cluster

A Di Gregorio et al. Gene. .

Abstract

In order to isolate genes important in controlling embryonic development in Tunicates, a genomic library from the ascidian Ciona intestinalis was screened with a degenerate oligodeoxyribonucleotide encoding the third helix of Antennapedia-type homeoboxes. Fourteen C. intestinalis homeobox genes, corresponding to several classes of homeodomains, have been identified. Five of the isolated homeoboxes show their highest homology to members of the Vertebrate HOX clusters. mRNAs for two of the isolated homeoboxes are present in unfertilized C. intestinalis eggs.

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