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. 1995 May 23;92(11):5164-8.
doi: 10.1073/pnas.92.11.5164.

Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces

W Cai  1 , H Aburatani, V P Stanton Jr, D E Housman, Y K Wang, D C Schwartz
Affiliations

Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces

W Cai et al. Proc Natl Acad Sci U S A. .

Abstract

We have developed a surface mounting technology for the rapid construction of ordered restriction maps from individual DNA molecules. Optical restriction maps constructed from yeast artificial chromosome DNA molecules mounted on specially derivatized glass surfaces are accurate and reproducible, and the technology is amenable to automation. The mounting procedures described here should also be useful for fluorescence in situ hybridization studies. We believe these improvements to optical mapping will further stimulate the development of nonelectrophoretic approaches to genome analysis.

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References

    1. Nucleic Acids Res. 1976 Sep;3(9):2387-98 - PubMed
    1. Methods Enzymol. 1976;44:19-45 - PubMed
    1. J Mol Biol. 1978 May 25;121(3):311-26 - PubMed
    1. Nature. 1983 Sep 15-21;305(5931):189-93 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 - PubMed

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