Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jun 2;268(5215):1312-8.
doi: 10.1126/science.7761851.

Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase

J M Bolduc  1 , D H Dyer, W G Scott, P Singer, R M Sweet, D E Koshland Jr, B L Stoddard
Affiliations
Free article

Mutagenesis and Laue structures of enzyme intermediates: isocitrate dehydrogenase

J M Bolduc et al. Science. .
Free article

Erratum in

  • Science 1995 Oct 20;270(5235):365

Abstract

Site-directed mutagenesis and Laue diffraction data to 2.5 A resolution were used to solve the structures of two sequential intermediates formed during the catalytic actions of isocitrate dehydrogenase. Both intermediates are distinct from the enzyme-substrate and enzyme-product complexes. Mutation of key catalytic residues changed the rate determining steps so that protein and substrate intermediates within the overall reaction pathway could be visualized.

PubMed Disclaimer

Publication types