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Comparative Study
. 1995 May 25;210(3):1025-30.
doi: 10.1006/bbrc.1995.1759.

Peroxynitrite causes DNA nicks in plasmid pBR322

M G Salgo  1 , K Stone, G L Squadrito, J R Battista, W A Pryor
Affiliations
Comparative Study

Peroxynitrite causes DNA nicks in plasmid pBR322

M G Salgo et al. Biochem Biophys Res Commun. .

Abstract

Peroxynitrite causes single-strand breaks in pBR322 supercoiled DNA as evidenced by agarose gel electrophoresis analysis. The effect of three free radical scavengers, namely mannitol, benzoate and dimethylsulfoxide, were studied. Mannitol failed to protect DNA from damage by peroxynitrite while benzoate and dimethylsulfoxide amplified the damage. These results suggest the damage caused by peroxynitrite alone is not mediated by free radicals since typical free radical scavengers fail to prevent the damage.

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