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. 1993 Aug;17(2):61-4.
doi: 10.1111/j.1472-765x.1993.tb00371.x.

Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coli

P Brigidi  1 , F Bolognani, M Rossi, C Cerre, D Matteuzzi
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Cloning of the gene for cholesterol oxidase in Bacillus spp., Lactobacillus reuteri and its expression in Escherichia coli

P Brigidi et al. Lett Appl Microbiol. 1993 Aug.

Abstract

The cloning of the cholesterol oxidase gene in several Gram-positive bacteria, including Lactobacillus reuteri of intestinal origin, was obtained. Only the transformants of Escherichia coli harbouring the recombinant plasmid pCHOA showed a good intracellular enzyme activity. The heterologous gene was stably maintained in Gram-positive transformants but no enzyme activity was detected.

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