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. 1993 Aug;57(8):1396-7.
doi: 10.1271/bbb.57.1396.

Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7

H Tsujibo  1 , H Orikoshi, C Imada, Y Okami, K Miyamoto, Y Inamori
Affiliations
Free article

Site-directed mutagenesis of chitinase from Alteromonas sp. strain O-7

H Tsujibo et al. Biosci Biotechnol Biochem. 1993 Aug.
Free article

Abstract

Chitinase (Chi85) from Alteromonas sp. strain O-7 contains the two conserved regions common to microbial and plant chitinases. We did site-directed mutagenesis of Chi85 to investigate the effects of the conserved amino acid residues on chitinase activity. We suggest that Asp-290 and Glu-292 of Chi85 may be the essential amino acid residues for the cleavage of beta-glycosidic linkage of chitin.

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