Separation and isolation of viable bacteria by capillary zone electrophoresis

Abstract

Mixtures of bacteria, Enterococcus faecalis (ATCC 29212), Streptococcus pyogenes (ATCC 19615), Streptococcus agalactiae (ATCC 13813), Streptococcus pneumoniae (ATCC 6303), and Staphylococcus aureus (ATCC 29213), were resolved into discrete electrophoretic bands using capillary zone electrophoresis (CZE). Bacteria remained viable (> 90%) during the electrophoretic process. Analysis of peak separation indicates organisms were quantitatively resolved and recovered at greater than 98% purity. E. faecalis was resolved into two discrete fractions with different chain assemblages that may reflect different developmental stages. These findings suggest that CZE can afford the microbiologist a new tool for studying the composition and distribution of microorganisms in mixed populations.