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. 1993;191(4):524-31.
doi: 10.1007/BF00195754.

Repeat units from a maize rDNA external spacer region exhibit DNA curvature and interact with high-mobility-group proteins

E A Griess  1 , K D Grasser, G Feix
Affiliations

Repeat units from a maize rDNA external spacer region exhibit DNA curvature and interact with high-mobility-group proteins

E A Griess et al. Planta. 1993.

Abstract

The 3-kb external spacer from a maize (Zea mays L. cv. A619) nuclear rRNA gene unit which contains nine highly homologous 200-bp repeat elements was found to include a region with DNA-curvature properties. The centre of curvature was localized within repeats 5 and 6 using a circular permutation assay. A 60-bp-long subfragment of this region was found to interact with nuclear proteins, including high-mobility-group (HMG) proteins, and with the maize HMGa protein synthesized in Escherichia coli from a recombinant plasmid. The potential influence of the binding of the HMG proteins on the conformation of this subfragment was studied with a permutation assay based on a bending vector.

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