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. 1993 Dec;17(6):297-9.
doi: 10.1111/j.1472-765x.1993.tb01471.x.

SbvI restriction endonuclease from Streptococcus bovis

I Vanat  1 , P Pristas, E Kutejov a, J Júdová, A Godány, P Javorský
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SbvI restriction endonuclease from Streptococcus bovis

I Vanat et al. Lett Appl Microbiol. 1993 Dec.

Abstract

Restriction endonuclease SbvI, an isoschizomer of HaeIII, has been isolated from rumen amylolytic bacterium Streptococcus bovis II/1. SbvI was purified from cell extract by phosphocellulose chromatography and heparin-Sepharose chromatography. The recognition sequence of SbvI was identified by digestion of pBR322, pUC9 and lambda-DNA and comparing the cleavage patterns obtained with computer-derived data. SbvI recognizes the 4-bp palindrome, 5'-GGCC-3' and cleaves DNA after the second G in the sequence, producing blunt ends.

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