Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Nov;40(2-3):361-4.
doi: 10.1007/BF00170393.

Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement

A Merckelbach  1 , S Gödecke, Z A Janowicz, C P Hollenberg
Affiliations

Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement

A Merckelbach et al. Appl Microbiol Biotechnol. 1993 Nov.

Abstract

The ura3 gene of Hansenula polymorpha was cloned, sequenced and used to generate a ura3 mutant from the wild-type strain of this yeast via integrative mutagenesis. The Tn5 neomycin-resistance marker (neo) under control of the ADH1 promoter from Saccharomyces cerevisiae served as a transformation marker. The results show that gene replacement can be achieved in H. polymorpha, a yeast with a high level of non-homologous integration.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed
    1. Nucleic Acids Res. 1985 May 10;13(9):3043-62 - PubMed
    1. Gene. 1989 Dec 14;84(2):303-9 - PubMed
    1. Yeast. 1991 Jul;7(5):431-43 - PubMed
    1. Nucleic Acids Res. 1980 Dec 11;8(23):5541-9 - PubMed

Substances

LinkOut - more resources