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. 1993 Nov;40(2-3):361-4.
doi: 10.1007/BF00170393.

Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement

A Merckelbach  1 , S Gödecke, Z A Janowicz, C P Hollenberg
Affiliations

Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement

A Merckelbach et al. Appl Microbiol Biotechnol. 1993 Nov.

Abstract

The ura3 gene of Hansenula polymorpha was cloned, sequenced and used to generate a ura3 mutant from the wild-type strain of this yeast via integrative mutagenesis. The Tn5 neomycin-resistance marker (neo) under control of the ADH1 promoter from Saccharomyces cerevisiae served as a transformation marker. The results show that gene replacement can be achieved in H. polymorpha, a yeast with a high level of non-homologous integration.

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