Review
doi: 10.1016/0167-7799(94)90080-9.
Engineering proteins to facilitate bioprocessing
Affiliations
- PMID: 7764901
- DOI: 10.1016/0167-7799(94)90080-9
Item in Clipboard
Review
Engineering proteins to facilitate bioprocessing
Trends Biotechnol.
1994 May.
Abstract
Genetic engineering is now being applied to aid the purification of recombinant proteins. The addition of specifically designed tags or the modification of sequences within the target-gene product has enabled the development of novel strategies for downstream processing that can be employed for efficient recovery of both native or modified proteins. This article discusses novel trends in genetic engineering that aid the bioprocessing of recombinant proteins.
Similar articles
-
Engineering proteins with novel mechanical properties by recombination of protein fragments.Angew Chem Int Ed Engl. 2006 Aug 25;45(34):5633-8. doi: 10.1002/anie.200600382. Angew Chem Int Ed Engl. 2006. PMID: 16856192 No abstract available.
-
Linkers for improved cleavage of fusion proteins with an engineered alpha-lytic protease.Biotechnol Bioeng. 2001 Aug 20;74(4):335-43. Biotechnol Bioeng. 2001. PMID: 11410858
-
Applications of novel affinity cassette methods: use of peptide fusion handles for the purification of recombinant proteins.J Mol Recognit. 2001 Nov-Dec;14(6):323-69. doi: 10.1002/jmr.555. J Mol Recognit. 2001. PMID: 11757069 Review.
-
Current status of technical protein refolding.J Biotechnol. 2007 Feb 20;128(3):587-96. doi: 10.1016/j.jbiotec.2006.12.004. Epub 2006 Dec 12. J Biotechnol. 2007. PMID: 17222934 Review.
-
Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins.Protein Expr Purif. 2006 Jul;48(1):1-13. doi: 10.1016/j.pep.2005.12.002. Epub 2005 Dec 28. Protein Expr Purif. 2006. PMID: 16427311 Review.
Cited by
-
Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused.Protein Sci. 1999 Aug;8(8):1668-74. doi: 10.1110/ps.8.8.1668. Protein Sci. 1999. PMID: 10452611 Free PMC article.
-
RNA affinity tags for the rapid purification and investigation of RNAs and RNA-protein complexes.Methods Mol Biol. 2008;488:23-40. doi: 10.1007/978-1-60327-475-3_3. Methods Mol Biol. 2008. PMID: 18982282 Free PMC article.
-
A family of LIC vectors for high-throughput cloning and purification of proteins.Methods Mol Biol. 2009;498:105-15. doi: 10.1007/978-1-59745-196-3_7. Methods Mol Biol. 2009. PMID: 18988021 Free PMC article.
-
Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion--tag.Microb Cell Fact. 2010 Nov 23;9:92. doi: 10.1186/1475-2859-9-92. Microb Cell Fact. 2010. PMID: 21092285 Free PMC article.
-
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors.Microb Cell Fact. 2005 Dec 13;4:34. doi: 10.1186/1475-2859-4-34. Microb Cell Fact. 2005. PMID: 16351710 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
