Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1994 Jun 30;35(2-3):109-20.
doi: 10.1016/0168-1656(94)90030-2.

Laser micromanipulators for biotechnology and genome research

N Ponelies  1 , J Scheef, A Harim, G Leitz, K O Greulich
Affiliations
Review

Laser micromanipulators for biotechnology and genome research

N Ponelies et al. J Biotechnol. .

Abstract

The use of lasers for complete micromanipulation of metaphase chromosomes, cells and subcellular structures is reviewed. DNA probes from single microdissected chromosome segments can be prepared using Alu or Adaptor PCR. In plant biotechnology, laser microsurgery can be used to prepare non-enzymatically protoplasts from Medicago sativa. Microgravity can be simulated in the alga Chara by lifting intracellular gravity transmitting elements with the optical tweezers.

PubMed Disclaimer

LinkOut - more resources