Review
doi: 10.1016/0167-7799(94)90140-6.
YACs, BACs, PACs and MACs: artificial chromosomes as research tools
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- PMID: 7765076
- DOI: 10.1016/0167-7799(94)90140-6
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Review
YACs, BACs, PACs and MACs: artificial chromosomes as research tools
Trends Biotechnol.
1994 Jul.
Abstract
Yeast artificial chromosomes (YACs) have become essential research tools as they enable large fragments of DNA to be cloned. In order to overcome several disadvantages of YACs, including chimaerism and instability, several complementary bacterial artificial chromosome (BAC) vectors have been developed. More recently, attempts are being made to construct artificial chromosomes in mammalian cells (MACs).
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