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Comparative Study
. 1994 Sep;12(9):915-8.
doi: 10.1038/nbt0994-915.

Recombinant Bacillus thuringiensis crystal proteins with new properties: possibilities for resistance management

D Bosch  1 , B Schipper, H van der Kleij, R A de Maagd, W J Stiekema
Affiliations
Comparative Study

Recombinant Bacillus thuringiensis crystal proteins with new properties: possibilities for resistance management

D Bosch et al. Biotechnology (N Y). 1994 Sep.

Abstract

To obtain Bacillus thuringiensis crystal proteins with new properties and to identify the regions involved in insecticidal activity, we generated hybrid genes composed of cryIC and cryIE by in vivo recombination. Analysis of the hybrid proteins showed that domain III of CryIC is involved in the toxicity towards Spodoptera exigua and Mamestra brassicae. Transfer of this domain to CryIE, which is not active against these insects, resulted in a new protein with a broader activity. This hybrid protein binds to different receptors than CryIC, suggesting its use as an alternative for CryIC in resistance management programs.

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