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. 1994 Nov;19(5):357-8.
doi: 10.1111/j.1472-765x.1994.tb00474.x.

Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni

J R Gibson  1 , K Sutherland, R J Owen
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Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni

J R Gibson et al. Lett Appl Microbiol. 1994 Nov.

Abstract

DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the preservation of the DNA in a state suitable for restriction digestion and subsequent electrophoretic analysis.

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