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. 1994 Dec;58(12):2283-5.
doi: 10.1271/bbb.58.2283.

Site-directed mutagenesis of the Asp-197 and Asp-202 residues in chitinase A1 of Bacillus circulans WL-12

T Watanabe  1 , M Uchida, K Kobori, H Tanaka
Affiliations
Free article

Site-directed mutagenesis of the Asp-197 and Asp-202 residues in chitinase A1 of Bacillus circulans WL-12

T Watanabe et al. Biosci Biotechnol Biochem. 1994 Dec.
Free article

Abstract

The contribution of the Asp-197 and Asp-202 residues in chitinase A1 of Bacillus circulans WL-12 to the catalytic reaction was studied by site-directed mutagenesis of these residues. A kinetic analysis of the purified mutant chitinases suggests the involvement of both the Asp-197 and Asp-202 residues in the catalytic events of this enzyme, although the effects of mutations of Asp-197 were less severe than those of the other mutations.

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