Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994;15(1-3):103-9.
doi: 10.1007/BF00762384.

Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process

D Drapeau  1 , Y T Luan, J A Popoloski, D T Richards, D C Cohen, M S Sinacore, S R Adamson
Affiliations

Extracellular insulin degrading activity creates instability in a CHO-based batch-refeed continuous process

D Drapeau et al. Cytotechnology. 1994.

Abstract

In a batch-refeed continuous process involving a recombinant Chinese hamster ovary cell line, a brief upset was occasionally observed during which cell growth halted and cell viability dropped. This was found to be associated with depletion of insulin from the medium early during the affected passage. Insulin depletion was found to be primarily the result of insulin degrading activity released by the cells during the preceding passage.

PubMed Disclaimer

References

    1. Endocr Rev. 1988 Aug;9(3):319-45 - PubMed
    1. J Biol Chem. 1994 Jan 28;269(4):3010-6 - PubMed
    1. In Vitro Cell Dev Biol. 1985 Oct;21(10):588-92 - PubMed
    1. In Vitro Cell Dev Biol. 1986 Feb;22(2):66-74 - PubMed
    1. Science. 1987 Mar 20;235(4795):1504-8 - PubMed

LinkOut - more resources