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Comparative Study
. 1994;15(1-3):223-8.
doi: 10.1007/BF00762397.

Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines

D Cant  1 , J Barford, C Harbour, A Fletcher, N Packer, A Gooley
Affiliations
Comparative Study

Glycosylation and functional activity of anti-D secreted by two human lymphoblastoid cell lines

D Cant et al. Cytotechnology. 1994.

Abstract

Cell lines BTSN4 and BTSN5 were produced by the Epstein-Barr Virus (EBV) transformation of B-lymphocytes from the same human donor. Both secrete an anti-D monoclonal of the IgG1 subclass but these antibodies display vastly different effector activities. Specifically, anti-D from BTSN4 has a far greater activity in both monocyte- and lymphocyte-mediated ADCC reactions and causes a higher percentage of rosettes to be formed with monocyte-like U937 cells. This variation in functional activity is shown to coincide with changes in the structure of the sugar chains attached to the asparagine-297 site on the immunoglobulin heavy chain.

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