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Comparative Study
. 1994;15(1-3):65-71.
doi: 10.1007/BF00762380.

Growth and interferon-gamma production in batch culture of CHO cells

V Leelavatcharamas  1 , A N Emery, M al-Rubeai
Affiliations
Comparative Study

Growth and interferon-gamma production in batch culture of CHO cells

V Leelavatcharamas et al. Cytotechnology. 1994.

Abstract

The relationship between growth and interferon-gamma (IFN-gamma) production in the recombinant cell line CHO 320 was studied by varying the foetal calf serum (FCS) concentration. The specific growth rate varied with the initial FCS concentration in a manner which could be well fitted by the Monod model. The Ks and mu max-values were found to be 0.771% (v/v) serum and 0.031 h-1 respectively. The average specific IFN-gamma production rates during the exponential phase increased with increasing FCS concentration. A good correlation between specific production rate and specific growth rate was found in all phases of the culture except the lag phase and it was clearly demonstrated that IFN-gamma production was growth associated. Specific glucose and glutamine utilisation rates were inversely related to specific growth rates.

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