Biodegradation and sorption of polyaromatic hydrocarbons by Phanerochaete chrysosporium

Abstract

The ability of the white-rot fungus Phanerochaete chrysosporium (INA-12) to degrade various polynuclear aromatic hydrocarbons (PAH) was investigated. Under static, non-nitrogen-limiting conditions, P. chrysosporium mineralized both phenanthrene and benzo[a]pyrene. Total mineralization, based on radioactive tracing, was limited to 1.8%-3% for phenanthrene and benzo[a]pyrene respectively. In both cases the pattern of mineralization did not correlate temporally with the production of lignin peroxidase activity. Sorption of radiolabelled material to the biomass was very significant with 22% and 40% of the total radioactivity being sorbed for benzo[a]pyrene and phenanthrene respectively. A number of models were examined to predict the sorption isotherms, the best performance being obtained with a three-parameter empirical model. It is apparent that lignin peroxidase is not necessarily involved in the biodegradation of all PAH and that a significant factor in PAH biodegradation and/or disappearance in cultures with the intact fungus may be attributed to sorption phenomena.