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. 1995 May 11;1244(1):216-22.
doi: 10.1016/0304-4165(95)00012-z.

Molecular cloning and expression of chick Gal beta 1,3GalNAc alpha 2,3-sialyltransferase

N Kurosawa  1 , T Hamamoto, M Inoue, S Tsuji
Affiliations

Molecular cloning and expression of chick Gal beta 1,3GalNAc alpha 2,3-sialyltransferase

N Kurosawa et al. Biochim Biophys Acta. .

Abstract

A cDNA clone encoding chick Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3Gal I) was isolated from a chick embryo brain cDNA library. The cDNA sequence included an open reading frame coding for 342 amino acids, and the deduced amino acid sequence showed 64% identity with that of the mouse enzyme. Northern blot analysis of chick embryos revealed that the ST3Gal I gene was expressed in early embryonic stages. The identity of the enzyme was confirmed by construction of a recombinant sialyltransferase in which the N-terminal part including the cytoplasmic tail and signal anchor domain was replaced with an immunoglobulin signal peptide sequence. This enzyme expressed in COS-7 cells exhibited transferase activity similar to that of mouse ST3Gal I.

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