Separation of r-hirudin from similar substances by capillary electrophoresis
- PMID: 7767465
- DOI: 10.1016/0021-9673(94)01261-c
Separation of r-hirudin from similar substances by capillary electrophoresis
Abstract
The thrombin inhibitor r-hirudin is a peptide of 65 amino acids and with a pI of 4.4. Capillary electrophoresis (CE) was used to separate r-hirudin from seven closely related substances which may be found as by-products or from degradation. Possibly two of these substances differ only in an isoaspartyl instead of an aspartyl binding. A baseline separation was possible with an acetate buffer (pH 4.4, 60 mM) containing 0.3% (m/m) PEG 20,000 and 0.1 mM Zn2+. The possibilities to prevent wall adsorption are discussed.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous