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. 1995 Jun;177(12):3619-22.
doi: 10.1128/jb.177.12.3619-3622.1995.

Topoisomerase activity during the heat shock response in Escherichia coli K-12

R Camacho-Carranza  1 , J Membrillo-Hernández, J Ramírez-Santos, J Castro-Dorantes, V Chagoya de Sánchez, M C Gómez-Eichelmann
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Topoisomerase activity during the heat shock response in Escherichia coli K-12

R Camacho-Carranza et al. J Bacteriol. 1995 Jun.

Abstract

During the upshift of temperature from 30 to 42, 45, 47, or 50 degrees C, an increase in the level of supercoiling of a reporter plasmid was observed. This increase was present in groE and dnaK mutants but was inhibited in cells treated with chloramphenicol and novobiocin. The intracellular [ATP]/[ADP] ratio increased rapidly after an upshift in temperature from 30 to 47 degrees C and then decreased to reach a level above that observed at 30 degrees C. These results suggest that gyrase and proteins synthesized during heat shock are responsible for the changes seen in plasmid supercoiling. Proteins GroE and DnaK are probably not involved in this phenomenon.

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References

    1. J Mol Biol. 1972 Nov 14;71(2):127-47 - PubMed
    1. Mol Microbiol. 1993 Nov;10(3):473-81 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4280-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4474-8 - PubMed

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