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. 1995 Mar;52(1-2):231-6.
doi: 10.1016/0166-0934(94)00131-y.

Development of a quantal assay in primary shrimp cell culture for yellow head baculovirus (YBV) of penaeid shrimp

Y Lu  1 , L M Tapay, P C Loh, J A Brock, R Gose
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Development of a quantal assay in primary shrimp cell culture for yellow head baculovirus (YBV) of penaeid shrimp

Y Lu et al. J Virol Methods. 1995 Mar.

Abstract

A 50% tissue culture infectious dose assay (TCID50) using primary culture of shrimp lymphoid organ (Oka) cells was developed for the quantitative titration of yellow-head baculovirus (YBV), a newly isolated virus of penaeid shrimp. The assay protocol includes the use of Primaria-grade 96-well tissue culture plates to grow the primary lymphoid organ cells of penaeid shrimp. A 15% gill suspension from YBV-infected shrimp was determined to have an infectious virus titer of 5 x 10(5.75) TCID50/ml. This report represents the first convenient assay protocol using cell culture derived from penaeid shrimp to titer a shrimp virus.

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