A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer
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- PMID: 7779401
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A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer
Biotechniques.
1995 Mar.
Erratum in
- Biotechniques 1995 Aug;19(2):212
Abstract
We describe a simple method for evaluating gap junctional communication (GJC) between cells in culture. The procedure involves pre-loading cells with two fluorescent dyes: calcein and DiI. Calcein is able to pass through gap junctions, while DiI is not. These pre-loaded cells are then plated with unlabeled cells. The number of cells receiving calcein from each pre-loaded cell can then be quantified after the cells settle on the plate. Potent and reversible inhibitors of GJC can be used in this system to evaluate dye transfer within a given period of time.
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