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. 1995 Apr;39(4):899-905.
doi: 10.1128/AAC.39.4.899.

SHV-7, a novel cefotaxime-hydrolyzing beta-lactamase, identified in Escherichia coli isolates from hospitalized nursing home patients

P A Bradford  1 , C Urban, A Jaiswal, N Mariano, B A Rasmussen, S J Projan, J J Rahal, K Bush
Affiliations

SHV-7, a novel cefotaxime-hydrolyzing beta-lactamase, identified in Escherichia coli isolates from hospitalized nursing home patients

P A Bradford et al. Antimicrob Agents Chemother. 1995 Apr.

Erratum in

  • Antimicrob Agents Chemother 1995 Jul;39(7):1646

Abstract

Four ceftazidime-resistant Escherichia coli strains were isolated from elderly nursing home patients in a New York hospital during 1993. Strains MCQ-2, MCQ-3, and MCQ-4 were determined to be identical by pulsed-field gel electrophoresis and plasmid profiles, whereas strain MCQ-1 was unique. Strain MCQ-1 was determined to produce a TEM-10 beta-lactamase. Strains MCQ-2, MCQ-3, and MCQ-4 were also noted to be resistant to cefotaxime. These three strains produced two beta-lactamases with pIs of 5.4 (TEM-1) and 7.6. beta-Lactamase assays revealed that the pI 7.6 enzyme hydrolyzed cefotaxime faster (at a relative hydrolysis rate of 30% compared with that of benzylpenicillin) than either ceftazidime or aztreonam (relative hydrolysis rates of 13 and 3.3%, respectively). Nucleotide sequencing of the gene encoding the pI 7.6 beta-lactamase from strain MCQ-3 revealed a blaSHV-type gene differing from the gene encoding SHV-1 at four nucleotides which resulted in amino acid substitutions: phenylalanine for isoleucine at position 8, serine for arginine at position 43, serine for glycine at position 238, and lysine for glutamate at position 240. This novel SHV-type extended-spectrum beta-lactamase is designated SHV-7.

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References

    1. J Gen Microbiol. 1975 May;88(1):169-78 - PubMed
    1. Antimicrob Agents Chemother. 1994 Apr;38(4):761-6 - PubMed
    1. Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 - PubMed
    1. Antimicrob Agents Chemother. 1982 Jan;21(1):85-92 - PubMed
    1. Antimicrob Agents Chemother. 1985 Aug;28(2):302-7 - PubMed

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