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. 1995 Apr;39(4):906-9.
doi: 10.1128/AAC.39.4.906.

Detection of amphotericin B-resistant Candida isolates in a broth-based system

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Detection of amphotericin B-resistant Candida isolates in a broth-based system

J H Rex et al. Antimicrob Agents Chemother. 1995 Apr.

Abstract

Because of the limited ability of the National Committee for Clinical Laboratory Standards proposed M27P methodology to detect resistance to amphotericin B by Candida isolates, we sought to identify alternative media and pH conditions that could reliably identify resistant isolates. Antibiotic Medium 3 broth (also known as Penassay broth) buffered to pH 5 or pH 7 produced superior results and readily identified a series of resistant isolates.

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