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. 1976 Jun;35(3):423-8.
doi: 10.1016/0027-5107(76)90204-9.

Indirect selection for auxotrophic mutants of Saccharomyces cerevisiae using the antibiotic netropsin

J D Young, J W Gorman, J A Gorman, R M Bock

Indirect selection for auxotrophic mutants of Saccharomyces cerevisiae using the antibiotic netropsin

J D Young et al. Mutat Res. 1976 Jun.

Abstract

The small basic oligopeptide antibiotic, netropsin, can be successfully employed as an effective counterselecting agent in Saccharomyces cerevisiae. The use of the drug results in approximately a 35-fold enrichment of auxotrophic mutants in a mutagenized culture of yeast. The experimental procedure is quite simple and less time consuming than other presently used methods for indirect mutant selection in yeast.

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