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. 1995 May 26;158(1):139-40.
doi: 10.1016/0378-1119(95)00093-l.

Isolation and characterization of a new restriction endonuclease, Sru30DI, from Selenomonas ruminantium

P Pristas  1 , I Vanat, P Javorský
Affiliations

Isolation and characterization of a new restriction endonuclease, Sru30DI, from Selenomonas ruminantium

P Pristas et al. Gene. .

Abstract

The restriction endonuclease (ENase) Sru30DI, an isoschizomer of StuI, which recognizes the sequence 5'-AGG/CCT-3', was purified from a natural isolate of Selenomonas ruminatinum. The ENase was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. Activity of Sru30DI is inhibited by overlapping Dcm methylation. The ENase is extremely stable at 37 degrees C and is active over a wide range of pH, temperature and salt concentrations.

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