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. 1978 May;26(2):536-9.
doi: 10.1128/JVI.26.2.536-539.1978.

Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries

P L Ratner, M L Cleary, E James

Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries

P L Ratner et al. J Virol. 1978 May.

Abstract

Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.

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