Rapid detection and isolation of mycoplasmas from cell cultures
- PMID: 779741
- DOI: 10.1038/icb.1975.28
Rapid detection and isolation of mycoplasmas from cell cultures
Abstract
An improved medium for the isolation of Mycoplasma species from contaminated cell cultures is reported. A modification of the mycoplasma detection method using the uridine/uracil uptake ratio method is described. Results obtained using this method with mycoplasma contaminated cell cultures and with contaminated cell cultures treated with the antibiotic Lincomycin are presented.
Similar articles
-
Comparative studies between microbiological culture and uptake of uridine/uracil to detect mycoplasmal infection of cell cultures.Exp Cell Res. 1979 Jun;121(1):159-65. doi: 10.1016/0014-4827(79)90456-7. Exp Cell Res. 1979. PMID: 376320 No abstract available.
-
Decontamination of mycoplasma-contaminated Orientia tsutsugamushi strains by repeating passages through cell cultures with antibiotics.BMC Microbiol. 2013 Feb 8;13:32. doi: 10.1186/1471-2180-13-32. BMC Microbiol. 2013. PMID: 23394970 Free PMC article.
-
A simple biochemical technique for the detection of mycoplasma contamination of cultured cells.Methods Cell Biol. 1975;10:277-90. doi: 10.1016/s0091-679x(08)60742-6. Methods Cell Biol. 1975. PMID: 1186505 No abstract available.
-
Detection of mycoplasmas in cell cultures by cultural methods.Methods Mol Biol. 1998;104:207-15. doi: 10.1385/0-89603-525-5:207. Methods Mol Biol. 1998. PMID: 9711656 Review. No abstract available.
-
Detection of mycoplasmas in cell cultures by fluorescence methods.Methods Mol Biol. 1998;104:217-26. doi: 10.1385/0-89603-525-5:217. Methods Mol Biol. 1998. PMID: 9711657 Review. No abstract available.
Cited by
-
Normal hydroxylation of proline in collagen synthesized by skin fibroblasts from a patient with prolidase deficiency.J Inherit Metab Dis. 1982;5(2):111-3. doi: 10.1007/BF01800003. J Inherit Metab Dis. 1982. PMID: 6820420
-
Purine synthesis de novo in cultured lymphoblast cells derived from patients with gout.Rheumatol Int. 1987;7(1):1-6. doi: 10.1007/BF00267335. Rheumatol Int. 1987. PMID: 3589399
-
Genetics of mammalian phenylalanine hydroxylase system. IV. Evidence of phenylalanine hydroxylase in a cultured human hepatoma cell line.Biochem Genet. 1980 Oct;18(9-10):955-68. doi: 10.1007/BF00500128. Biochem Genet. 1980. PMID: 7194638
-
Some in-vitro comparisons of synovial cells dispersed by trypsin from rheumatoid and nonrheumatoid synovium.Ann Rheum Dis. 1981 Jun;40(3):286-92. doi: 10.1136/ard.40.3.286. Ann Rheum Dis. 1981. PMID: 7247472 Free PMC article.
-
Reduced lysyl oxidase activity in skin fibroblasts from patients with Menkes' syndrome.Biochem J. 1980 Nov 15;192(2):579-86. doi: 10.1042/bj1920579. Biochem J. 1980. PMID: 6112984 Free PMC article.