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. 1994 Sep;44(1):54-8.
doi: 10.1002/jmv.1890440111.

Rapid detection of virus genome from imported dengue fever and dengue hemorrhagic fever patients by direct polymerase chain reaction

K Morita  1 , T Maemoto, S Honda, K Onishi, M Murata, M Tanaka, A Igarashi
Affiliations

Rapid detection of virus genome from imported dengue fever and dengue hemorrhagic fever patients by direct polymerase chain reaction

K Morita et al. J Med Virol. 1994 Sep.

Abstract

Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT-PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. The results of PCR corresponded closely to the results of virus isolation using cultured mosquito cells, suggesting that direct RT-PCR procedure greatly facilitates rapid diagnosis of dengue infection.

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