Nucleotide sequence of the petE gene encoding plastocyanin from the photosynthetic prokaryote, Prochlorothrix hollandica

Abstract

We have determined the nucleotide sequence of the petE gene encoding plastocyanin from the chlorophyll a/b-containing photosynthetic prokaryote, Prochlorothrix hollandica. Comparison of the deduced amino acid sequence encoded by the gene with the N-terminal sequence of the purified protein revealed that plastocyanin is synthesized as a precursor bearing an N-terminal domain of 34 amino acids having some structural similarity to thylakoid lumenal transit peptides identified in other organisms. The mature protein has an apparent isoelectric point of 8.37 and a molecular mass of 10,236 Da.