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. 1994 Dec 19;356(2-3):342-4.
doi: 10.1016/0014-5793(94)01313-6.

Characterization and determination of the redox properties of the 2[4Fe-4S] ferredoxin from Methanosarcina barkeri strain MS

P J Daas  1 , W R Hagen, J T Keltjens, G D Vogels
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Free article

Characterization and determination of the redox properties of the 2[4Fe-4S] ferredoxin from Methanosarcina barkeri strain MS

P J Daas et al. FEBS Lett. .
Free article

Abstract

Ferredoxin was purified from methanol-grown Methanosarcina barkeri strain MS. It was isolated as a dimer with a subunit molecular weight of 6,200. The protein contained 7.4 mol iron and 7.2 mol acid-labile sulfur per monomer. In the reduced state the ferredoxin exhibited an EPR spectrum characteristic of two spin-coupled [4Fe-4S]1+ clusters. The EM of the [4Fe-4S]2+:1+ couple was -322 mV +/- 3 mV vs. NHE at 21 degrees C and pH 7.0. The midpoint potential was temperature but not pH dependent. At the physiological temperature of 37 degrees C the Em was -340 mV.

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